Figure 2.

v-Src overrides the spindle assembly checkpoint. HeLa S3/v-Src cells were treated with 10 μm STLC with or without 2 ng/ml Dox for 24 h and fixed with 4% formaldehyde in PBS(−) for 20 min at room temperature. A, fixed cells were stained for phospho-Src at Tyr-416 (Src pY416, red), lamin A/C (green), and DNA (blue). Arrows indicate interphase cells expressing v-Src. B, cells were stained for cyclin B1 (red), Src (green), and DNA (blue). Arrows (pink) and arrowheads (blue) indicate interphase cells with low and high expression levels of cyclin B1, respectively. C, cells were stained for cyclin B1 (red), lamin A/C (green), and DNA (blue). Arrows (pink) and arrowheads (blue) indicate interphase cells with low and high expression levels of cyclin B1, respectively. Scale bars, 20 μm.