Figure 1.

Yeast two-hybrid interaction assay of Pex5p and Pex1p–Pex6p. The S. cerevisiae strain PJ69-4A was transformed with proteins of interest fused to either the activation (AD) or binding domain (BD) of Gal4p as indicated. Interactions were monitored by histidine/adenine auxotrophy. Neither Pex1p nor Pex6p show an interaction with Pex5p under these conditions. The controls comprise the known interactions Pex5p–Pex14p, Pex1p–Pex6p, and Pex15p(1–315)-Pex6p, which confirm that all constructs are expressed and folded correctly.