Figure 7.
Effect of delivering zymosan-bound SOD to neutrophil phagosomes. Neutrophils (5 × 106/ml) were pretreated with either 10 μm dihydroethidium (gray bars) or 10 μm R19-S (black bars), and then incubated with the zymosan preparations (5 × 107/ml) for 30 min at 37 °C. Mean intracellular fluorescence of the neutrophils was measured by flow cytometry, and values are expressed relative to the fluorescence with normal zymosan. *, dihydroethidium fluorescence significantly decreased and R19 fluorescence significantly increased with SOD-linked zymosan compared with phagocytosis of normal zymosan or inactive SOD-zymosan (p < 0.01, paired t test). Results are mean ± S.D. from three separate experiments.