Figure 2.

Spectral analysis of purified recombinant D2HGDH. A, the mixture containing 20 mm Tris-HCl (pH 8.0), 5 μm ZnCl₂, and purified D2HGDH (1 mg ml⁻¹) was flushed for 5 min with N₂ in a quartz glass cuvette. The absorbance spectra were recorded at 30 °C. B and C, after addition of d-2-HG (B) and after addition of d-malate (C) at a final concentration of 250 μm, the changes of absorbance spectra were followed over different times using a UV-visible spectrophotometer. d-MT, d-malate.