Figure 3.

Effect of metal ions on the activity of the D2HGDH toward d-2-HG and d-malate. The d-2-HG dehydrogenase activities (A) and d-malate dehydrogenase activities (B) of D2HGDH were assayed spectrophotometrically in the absence or presence of 10 μm or 10 mm of the chloride salts of the indicated metal ions and with 1 mm d-2-HG (A) and 10 mm d-malate (B) as substrates. The effect of EDTA at a final concentration of 1 mm was also tested. Control reactions without metal ion were run for background correction. The values shown correspond to means ± S.D. from three independent replicates.