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D2HGDH coupled with ETF for efficient oxidization of d-malate. The effect of ETF (0–4.25 μm) on D2HGDH-catalyzed DCIP (100 μm) reduction in the presence of a fixed concentration of d-malate (2.5 mm) was assayed. The reaction mixtures with no D2HGDH (red line) and no d-malate (black line) were taken as control.
