Table 1.

Steady-state kinetic parameters of SerA toward different substrates

The reactions with the reported kinetic parameters of SerA from several representative species are listed. NR, not reported.

Reaction	Km	Vmax	kcat	kcat/Km
	mm	units mg−1		s−1 m−1
SerA of P. stutzeria
2-KG reduction	0.13 ± 0.01	2.59 ± 0.03	7.75 ± 0.13 s−1	(5.81 ± 0.41) × 104
OAA reduction	1.10 ± 0.02	0.19 ± 0.01	0.57 ± 0.01 s−1	(5.17 ± 0.08) × 102
Pyruvate reduction	8.62 ± 0.85	(3.56 ± 0.13) × 10−2	0.11 ± 0.01 s−1	12.49 ± 0.91
SerA of E. coli
2-KG reductionb	0.042	NR	7.7 s−1	1.8 × 105
2-KG reductionc	0.088	11,100	33.3 s−1	3.8 × 105
3-PG oxidationc	1.2	183	0.55 s−1	5.0 × 102
3-PHP reductionc	0.0032	9,270	27.8 s−1	8.7 × 106
SerA of Mycobacterium tuberculosis
3-PHP reductiond	(170 ± 50) × 10−3	NR	2461 ± 281 s−1	1.5 × 107
SerA of Homo sapiens
3-PHP reductione	(127 ± 66) × 10−3	NR	311 ± 55 s−1	0.24 × 107
3-PHP reductionf	(3 ± 1) × 10−3	NR	41 ± 5 s−1	1.4 × 107
3-PG oxidationg	0.26 ± 0.034	NR	0.18 ± 0.01 min−1	NR
2-KG reductiong	10.1 ± 1.8	NR	4.7 ± 0.9 min−1	NR
OAA reductiong	6.5 ± 1.3	NR	10.6 ± 1.6 min−1	NR

^a The values are the means ± S.D. (n = 3).

^b Data from Ref. 13.

^c Data from Ref. 14.

^d Data from Ref. 15.

^e Data from Ref. 16, determined in 200 mm KPO₄ buffer.

^f Data from Ref. 16, determined in 50 mm MOPS buffer.

^g Data from Ref. 17.