Table 2.

Steady-state kinetic parameters of D2HGDH toward different substrates

The reactions with the reported kinetic parameters of D2HGDHs from several representative species are listed. NR, not reported.

Substrate	Km	Vmax	kcat	kcat/Km
	mm	units mg−1	s−1	s−1 mm−1
D2HGDH of P. stutzeria
d-2-HGb	0.17 ± 0.02	4.56 ± 0.60	7.90 ± 1.05	45.39 ± 1.52
d-Malate	3.61 ± 0.14	6.87 ± 0.23	11.70 ± 0.39	3.25 ± 0.02
ETF	(4.71 ± 0.03) × 10−3	2.71 ± 0.03	4.62 ± 0.04	980.82 ± 9.95
D2HGDH of A. thalianac
d-2-HG	∼0.58	NR	∼0.8	∼1.37
Dld2 of S. cerevisiaed
d-2-HG	(28 ± 8) × 10−3	NR	0.18 ± 0.03	7.0 ± 1.2
Dld3 of S. cerevisiaed
d-2-HG	(130 ± 9) × 10−3	NR	6.6 ± 0.5	50 ± 2

^a The values are the means ± S.D. (n = 3).

^b Data from Ref. 4.

^c Data from Ref. 21.

^d Data from Ref. 22.