Table 4.

The specific activities of D2HGDH in P. stutzeri A1501 during growth in medium containing different carbon sources

The values are the means ± S.D. (n = 3). The initial concentration of carbon source is ∼4 g liter⁻¹.

Medium	d-Malatea			d-2-HGb
	WT	Δd2hgdh	Δd2hgdh-d2hgdh+	WT	Δd2hgdh	Δd2hgdh-d2hgdh+
	milliunits mg−1			milliunits mg−1
OAA	0.73 ± 0.01	Undetected	1.54 ± 0.04	3.39 ± 0.09	Undetected	2.45 ± 0.17
2-KG	1.61 ± 0.03	Undetected	0.74 ± 0.01	1.65 ± 0.09	Undetected	6.76 ± 1.79
Pyruvate	22.90 ± 6.42	Undetected	7.55 ± 3.67	26.66 ± 9.44	Undetected	16.33 ± 7.45
Glucose	16.25 ± 1.64	Undetected	1.44 ± 0.79	27.83 ± 2.54	Undetected	6.13 ± 0.35
d-2-HG	187.00 ± 0.13	Not determined	152.00 ± 9.83	64.23 ± 7.39	Not determined	46.43 ± 2.55
d-Malate	282.00 ± 9.98	Not determined	123.00 ± 6.58	80.97 ± 2.91	Not determined	36.15 ± 4.85

^a Activities were examined with 10 mm d-malate.

^b Activities were examined with 1 mm d-2-HG. DCIP was used as the electron acceptor.