Figure 3.

Chemotactic activity of P1 and P3 on neutrophils. Bone marrow neutrophils from WT, Fpr1, Fpr2, and Fpr1/2 KO mice were used to examine the chemotactic activity of P1 and P3 using an experimental setup similar to that described for the HEK293 cells. The results are expressed as the mean (±S.D.) of the chemotaxis index (CI), denoting the fold increase in migrated cells in response to stimulants versus control (C), which consisted of the medium used to perform the assay (cells responded in the presence of only the medium). The experiments were performed three times in triplicate, with a representative set of triplicates being shown. W peptide (W), a chemotactic peptide activating both Fpr1 and Fpr2, was used as a positive control. * denotes significantly increased neutrophil responses to P1 and P3 compared with the control (p < 0.05).