Figure 7.

Solution GAGs/surface heparin competition. A, sensorgrams of competition experiments between solution GAGs and surface heparin. The assays used 5 μm P1 and 1 μm GAGs in solution. B, bar graphs of normalized P1 binding preference to surface heparin when it competes with the indicated GAGs in solution. C, same as A but for P3. D, same as B but for P3. The experiments were performed in triplicate, with one representative data set displayed in A and C. The bar graph data in B and D represent the mean ± S.D. for the triplicate. Statistical analysis of the bar graph data in B and D shows p < 0.05 when CSE in solution is compared with the buffer control and p < 0.005 when heparin (HP) in solution is compared with the buffer control.