Table 3.
Identities of complete genome and ORFs between RdHEVAC and representative orthohepevirus reference strains
| Hepeviridae | Degree of identity (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Full genome | ORF1 | ORF2 | ORF3 | ORF4 | ||||||
| nt | nt | aa | nt | aa | nt | aa | nt | aa | ||
| Orthohepevirus A | HEV-1 | 51.2–51.6 | 49.3–49.4 | 48.3–48.6 | 55.6–56.3 | 55.4–55.8 | 39.6–40.9 | 29.4 | NA | NA |
| HEV-2 | 50.7–51.0 | 48.4–49.0 | 47.6–48.0 | 55.4 | 55.1–55.4 | 39.0–40.1 | 26.1–27.7 | NA | NA | |
| HEV-3 | 50.9–51.0 | 49.2–49.3 | 48.9–49.0 | 56.2–56.9 | 55.7.56.0 | 40.7–41.6 | 28.8 | NA | NA | |
| HEV-4 | 50.4–50.6 | 48.5–48.8 | 48.9–49.2 | 54.7–55.1 | 53.8–54.2 | 36.8–38.4 | 26.1–26.9 | NA | NA | |
| HEV-5 | 51.4–51.7 | 49.3–49.5 | 48.8–49.2 | 56.2–56.5 | 55.1–55.4 | 38.8–39.9 | 27.4–30.0 | NA | NA | |
| HEV-6 | 51.1–51.7 | 49.3–49.4 | 49.1–49.5 | 56.4–57.4 | 54.7–55.3 | 38.5–39.7 | 27.4–30.8 | NA | NA | |
| HEV-7 | 51.1–51.5 | 49.3 | 48.1–48.3 | 55.6–57.0 | 56.0 | 39.9–42.1 | 28.8 | NA | NA | |
| Orthohepevirus B | 45.6–45.8 | 46.4–46.8 | 43.2–43.3 | 45.7–45.8 | 40.6–40.9 | 25.5–26.1 | 16.4 | NA | NA | |
| Orthohepevirus C | HEV-C1 | 64.3–65.5 | 61.5–63.8 | 66.0–68.0 | 67.7–69.6 | 71.9–74.0 | 60.1–62.6 | 41.5–46.2 | 70.5–72.6 | 47.5–54.1 |
| HEV-C2 | 62.0–62.8 | 61.2–62.1 | 67.8–68.6 | 64.7–65.5 | 70.9–71.6 | 54.9–56.7 | 35.5–36.3 | 69.7–70.7 | 49.2–50.8 | |
| RdHEVEm | 56.4–56.8 | 52.4–52.7 | 51.6–53.4 | 61.2–62.0 | 62.2–62.8 | 45.5–45.8 | 23.5–25.2 | NA | NA | |
| Orthohepevirus D | 47.2–47.4 | 45.6–45.8 | 41.5–41.8 | 48.5–49.3 | 45.0–45.2 | 27.5–28.2 | 10.6–13.0 | NA | NA | |
| Piscihepevirus A | 36.1–36.3 | 36.8 | 24.2–24.6 | 31.3–31.5 | 15.6–15.9 | 18.3–19.1 | 11.4–12 | NA | NA | |
The sequences were aligned using MAFFT. The evolutionary analyses were conducted using MEGA version 7. The GenBank Accession numbers are as follows: M73218 for HEV-1; M74506 for HEV-2; AF082843 for HEV-3; AJ272108 for HEV-4; AB573435 for HEV-5; AB602441 for HEV-6; KJ496143 for HEV-7; AY535004 for Orthohepevirus B; AB847309, AB847305, AB847306, AB847308, AB847307, GU345043, GU345042, JN167537, and JN167538 for HEV-C1; AB890001, JN998607, and JN998606 for HEV-C2; JQ001749 for Orthohepevirus D; and HQ731075 for Piscihepevirus A. RdHEVAC14 and RdHEVAC86 for RdHEVAc; RdHEVEM40 and RdHEVEm67 for RdHEVEm. Nucleotide for nt; amino acid for aa; and not available for NA.