Table 4.

Identities of complete genome and ORFs between RdHEVEm and representative orthohepevirus reference strains

Hepeviridae		Degree of identity (%)
		Full genome	ORF1		ORF2		ORF3
		nt	nt	aa	nt	aa	nt	aa
Orthohepevirus A	HEV-1	50.7–51.0	49.9–50.1	46.9–47.6	55.2–55.3	53.6	40.2–40.7	20.9–23.3
	HEV-2	50.3–50.7	50.0–50.2	47.2–48.2	54.0–54.2	52.6	38.3–38.8	19.4–20.9
	HEV-3	50.4	50.1	47.2–48.2	55.4	52.6–52.7	40.5–40.8	20.3–22.7
	HEV-4	50.0	49.9–50.0	47.2–48.1	54.5–54.9	51.2–51.4	38.8–39.4	23.3–24.0
	HEV-5	50.8–51.0	50.3	47.0–47.8	55.0	52.8–52.9	40.3–40.5	19.7–20.5
	HEV-6	50.0–50.5	49.9–50.0	47.7–48.5	54.0–54.5	53.5	41.1	20.5–22.0
	HEV-7	50.8–51.2	50.4–51.0	47.6–48.4	54.5–54.8	54.5–54.7	41.6–42.7	20.3–21.1
Orthohepevirus B		44.8–45.0	46.5–46.6	40.7–41.5	45.4–45.5	40.1	23.2–23.8	11.6–12.4
Orthohepevirus C	HEV-C1	55.9–56.8	52.4–54.1	52.4–53.5	60.5–61.8	61.1–62.8	42.3–47.9	22.3–24.8
	HEV-C2	55.1–56.1	53.5–54.2	52.8–54.3	57.8–59.7	58.4–58.8	45.7–46.3	22.0–26.3
	RdHEVAc	56.4–56.8	52.4–52.7	51.6–53.4	61.2–62.0	62.2–62.8	45.5–45.8	23.5–25.2
Orthohepevirus D		46.7–46.9	47.7–47.9	41.2–41.8	48.0–48.2	44.1–44.2	27.7–27.9	17.1
Piscihepevirus A		34.6–35.0	36.1–36.4	24.5–24.9	30.7–30.9	16.7	17.5–17.9	11.4

The sequences were aligned using MAFFT. The evolutionary analyses were conducted using MEGA version 7. The GenBank Accession numbers are as follows: M73218 for HEV-1; M74506 for HEV-2; AF082843 for HEV-3; AJ272108 for HEV-4; AB573435 for HEV-5; AB602441 for HEV-6; KJ496143 for HEV-7; AY535004 for Orthohepevirus B; AB847309, AB847305, AB847306, AB847308, AB847307, GU345043, GU345042, JN167537, and JN167538 for HEV-C1; AB890001, JN998607, and JN998606 for HEV-C2; JQ001749 for Orthohepevirus D; and HQ731075 for Piscihepevirus A. RdHEVAC14 and RdHEVAC86 for RdHEVAc; RdHEVEM40 and RdHEVEm67 for RdHEVEm. Nucleotide for nt; amino acid for aa; and not available for NA.