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. 2018 Jun 29;33(4):314–322. doi: 10.1007/s12250-018-0040-3

Fig. 3.

Fig. 3

Knockdown of G3BP1 and/or TIA1 does not affect cell viability. A HeLa cells were co-transfected with pEGFP-G3BP1 and si-G3BP1 or pEGFP-TIA1 and si-TIA1 for 48 h. Control cells were co-transfected with pEGFP-G3BP1 and si-Control or pEGFP-TIA1 and si-Control (a and c). The expression of EGFP-G3BP1 and EGFP-TIA1 were determined with a fluorescence microscope (a and c). Fluorescence intensity was determined with a NanoDrop 3300 (b and d). B HeLa cells were transfected with either si-G3BP1 or si-TIA1, or siG3BP1 and si-TIA1 for 48 h. Control cells were treated with si-Control. Cell viability was determined using the MTT assay (n = 4).