Fig. 5.

Potential interaction sites in core protein are shown. (A) Schematic presentation of Core-HA mutants. (B) Western blot analysis was performed to detect the expression of the mutants. (C) Co-immunoprecipitation assay was performed to confirm the interaction between filamin B and core protein mutants. A filamin B plasmid was co-transfected with a wild-type or mutated core protein plasmid into HEK 293T cells. Filamin B was immunoprecipitated using GFP. Co-precipitated core protein was detected by immunoblotting with anti-HA antibodies (top). Western blot analysis of the co-precipitated filamin B and GFP is shown using anti-GFP antibodies (top). Cell lysates before immunoprecipitation were probed with anti-GFP antibodies (bottom).