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Construction and identification of recombinant pif-replaced HearNPV bacmids. EGFP and chloramphenicol resistance coding sequences were inserted within pif loci. Hapif promoters and mbpif coding regions were respectively sequentially inserted in conjunction with ph coding region into the ph locus within the HaBac∆pifs bacmid. Upon examination and identification of the constructed bacmids, they were labelled as HaBacΔpif1-Mbpif1, HaBacΔpif2-Mbpif2 and HaBacΔpif3-Mbpif3.
