Fig. 7.
Detection of PIF complex in recombinant HearNPVs. OBs were extracted from infected cells at 7 d.p.i. and ODVs were liberated from the OBs by DAS treatment, concentrated and resuspended in Laemmli buffer. The ODV samples were heat treated at 50 °C (50) or 95 °C (95) for 5 min prior to SDS-PAGE analysis. ODVs extracted from 1 × 107 OBs were loaded into each well of the PAGE gel. Further protein detection was done using primary polyclonal antibodies anti-HaPIF1, anti-HaPIF2 and anti-HaVP91 for detecting PIF complex, and anti-HaGP41 for detecting ODV tegument protein GP41.