Figure 1.

Blocking of PLK4 or STIL Leads to Centrosome Loss Followed by Decreased Proliferation of Stem Cells

(A and B) Immunofluorescence (A) of 3-day vehicle- and centrinone-treated hESCs: centrosomes were visualized by antibody staining of distal marker CP110 (green) and proximal marker Cep135 (red). Scale bars, 1 μm. (B) Quantification of centrosome depletion, N > 150.

(C and D) Growth curves: cell number was measured at indicated time points by crystal violet assay, in vehicle- and centrinone-treated cells (C) or after STIL shRNA transfection (D).

(E) Western blot analyses of Ki-67 expression in 4-day vehicle- and centrinone-treated cells, with α-tubulin as a loading control.

Data are presented as mean ± SEM (^∗p < 0.05, ^∗∗p < 0.005, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001). See also Figure S1.