Figure 2.

Hematopoietic Reconstitution of Gene Therapy-Treated th3/+ Mice Is Highly Polyclonal and Dynamic

DNA extracted from in vitro cultures, peripheral blood at 16 weeks, and bone marrow at termination was analyzed for vector integration. (A) Venn diagram showing the overlapping ISs between PB and BM. (B) Shannon diversity index values (y axis) in in vitro samples, PB, and BM. (C) Histograms of the percentage of sequence reads (y axis) for unequivocally mapped ISs from PB. The number of reads for each IS is normalized to the total number of sequence reads from the same time point and for the engraftment level. ISs below 1% were collapsed and are shown in gray. (D) ISs in BM.