Figure 3.

N-Glycan Branching in the Stem Cell Niche Is High during Astrogenic Developmental Stages In Vivo

(A) L-PHA indicates highly branched N-glycans in sagittal sections of the cerebral cortex at E12 and E16. Arrowheads point to blood vessels, which were excluded from analysis. Dotted boxes indicate example regions used for quantitative analysis (10 boxes or more were analyzed per layer in each section). More intense staining is evident in the E16 NSPC niche (VZ/SVZ) than the E12 niche (VZ).

(B) Significantly greater staining in the E16 NSPC niche but no difference in the CP (VZ/SVZ E12 versus E16, p < 0.0001; CP E12 versus E16, p = 0.1041, unpaired Student's t test).

(C) L-PHA staining increases in the NSPC niche (VZ/SVZ) from E10 to E18 without a corresponding increase in the CP.

(D) Statistical analysis of the data in (C) indicates a significant increase in L-PHA intensity in the VZ/SVZ over time (p < 0.0001, one-way ANOVA, Tukey post hoc for multiple comparisons; ns, not significant).

Error bars SEM. N = 3 or more independent biological repeats (^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001).