Table 1. Primer pairs used in PCR experiments.
| Gene and accession number | Primer sequences (5′→3′) | Product (bp) |
|---|---|---|
| ACTIN |
s ATGGCCACGGCTGCTTCCAGC |
237 |
|
NM_001101 |
as CATGGTGGTGCCGCCAGACAG |
|
| AQP5 |
s ACTGGGTTTTCTGGGTAGGG |
184 |
|
NM_001651.2 |
as GTGGTCAGCTCCATGGTCTT |
|
| AQP8 |
s TCCTGAGGAGAGGTTCTGGA |
156 |
|
NM_001169.2 |
as AGGGCCCTTTGTCTTCTCAT |
|
| AR (AKR1B1) |
s CCCATGTGTACCAGAATGAGAAT |
190 |
|
NM_001628.2 |
as AGGTAGAGGTCCAGGTAGTCCAG |
|
| COX2 |
s TGAGCATCTACGGTTTGCTG |
158 |
|
NM_000963 |
as TGCTTGTCTGGAACAACTGC |
|
| bFGF (FGF2) |
s AGAGCGACCCTCACATCAAG |
234 |
|
NM_002006 |
as ACTGCCCAGTTCGTTTCAGT |
|
| IL1B |
s TGGGCCTCAAGGAAAAGAATC |
358 |
|
NM_000576.2 |
as CTTTCTGTTCCCTTTCTGCCA |
|
| HBEGF |
s TGCCTGTAGCTTTCCTGGTCCC |
258 |
|
NM_001945 |
as CCCCACCTCCAACCTTCTCGG |
|
| HIF1A |
s CACAGAAATGGCCTTGTGAA |
214 |
|
NM_001530.3 |
as CCAAGCAGGTCATAGGTGGT |
|
| NFAT5 |
s TCACCATCATCTTCCCACCT |
174 |
|
NM_006599.3 |
as CTGCAATAGTGCATCGCTGT |
|
| PlGF2 |
s GGCGATGAGAATCTGCACTGT |
164 |
|
NM_001207012.1 |
as CACCTTTCCGGCTTCATCTTC |
|
| NLRP3 |
s AGACAGCATTGAAGAGGAGTGG |
169 |
|
NM_183395.2 |
as TTTGTTGAGGCTCACACTCTCA |
|
| TGFB2 |
s ACGTCTCAGCAATGGAGAAGA |
195 |
|
NM_001135599.2 |
as ATTCGCCTTCTGCTCTTGTTT |
|
| VEGFA188, 164, 120 |
s CCTGGTGGACATCTTCCAGGAGTA |
479; 407; |
|
NM_003376.5, NM_001287044.1, NM_001025370.2 |
as CTCACCGCCTCGGCTTGTCACA |
275 |
| CFOS |
s AGAATCCGAAGGGAAAGGAA |
150 |
|
NM_005252.3 |
as CTTCTCCTTCAGCAGGTTGG |
|
| CJUN |
s CCCCAAGATCCTGAAACAGA |
168 |
|
NM_002228.3 |
as CCGTTGCTGGACTGGATTAT |
|
| JUNB |
s TGGAACAGCCCTTCTACCAC |
241 |
|
NM_002229.2 |
as GAAGAGGCGAGCTTGAGAGA |
|
| JUND |
s TTCTACTCGGGGAACAAACG |
213 |
|
NM_005354.5 |
as GGCGAACCAAGGATTACAAA |
|
| FOSB |
s GAGAAGGAACGTCTGGAGTTTGT |
227 |
|
NM_001114171.1 |
as GTAAAGAGAGAAGCCGTCAGGTT |
|
| FOSL1 |
s AAACTGGAAGATGAGAAATCTGG |
202 |
|
NM_001300857.1 |
as GGGAAAGGGAGATACAAGGTACA |
|
| FOSL2 |
s ACAGTGATCACCTCCATGTCC |
162 |
|
NM_005253.3 |
as AGACAGCTGCTCATCTCTCCTC |
|
| BATF |
s AGCGAAGACCTGGAGAAACA |
189 |
|
NM_006399.3 |
as GGAGCTGACATGAGGTTGGT |
|
| ATF2 |
s CCCACATCAGCTATTGTTCGT |
153 |
|
NM_001256094.1 |
as CACAATTGGCCTGTTAGAGGA |
|
| ATF3 |
s TTGAGGATTTTGCTAACCTGACG |
233 |
|
NM_001674.3 |
as CTTCTTCTTGTTTCGGCACTTTG |
|
| JDP1 |
s AGTTGCTGCTCAGAGAAGTCG |
167 |
|
NM_018664.2 |
as ACATCTTCTCGTGCTCCTTCA |
|
| JDP2 |
s TGACTGTGGAGGAGCTGAAATAC |
171 |
|
NM_001135049.1 |
as CTGCTGCGACTTTGTTCTTCT |
|
| CREB1 |
s AACAGAAGCTGAAAACCAACAAAT |
160 |
| NM_004379.4 | as ATGACTCCATGGACTTGAACTGT |
s, sense. as, anti-sense.