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Steady-state mRNA and protein expression levels for different LRRK2 mutants.
(A) Steady-state levels of protein expression of WT, G2385R, and G2385E LRRK2 in HEK293FT cells transiently transfected with an equal amount of 3xFLAG-HD-LRRK2 plasmid DNA. Quantification of protein expression levels (B), FLAG-tagged mRNA (C), and human LRRK2 mRNA (D) normalized to cyclophilin B protein and mRNA expression, respectively (n = 3 independent experiments).
