Figure 3.

Levels of WT, G2385R, and ΔC LRRK2 variant expression are inversely related to CHIP binding.

(A) Steady-state levels of protein expression of WT, G2385R, and ΔC LRRK2 (C-terminus-deleted LRRK2, containing amino acids 1–2498) in HEK293FT cells were transiently transfected with an equal amounts of plasmid DNA and pretreated for 3 h with DMSO or 1 μM GA prior to cell lysis. (B) CHIP-binding ability of different LRRK2 mutants in HEK293FT cells transfected with an adjusted amount of plasmid DNA in the absence (DMSO) or presence of 1 μM GA for 3 h. Quantifications of protein expression levels normalized to GAPDH (n = 3) (C) and CHIP, Hsc70, Hsp90, and ubiquitin binding (n = 6) (D–G) normalized to GAPDH. (E) Correlation analysis of steady-state protein expression levels and CHIP binding by different LRRK2 mutants.