Figure 7.

CHIP overexpression decreases protein expression levels of WT and G2385R LRRK2.

HEK293FT cells were transiently co-transfected with 1 μg each of either 3xFlag-HD-human LRRK2 WT or G2385R along with either 3xFlag-GFP or 2xmyc-CHIP plasmid. Cells were collected and lysed 40 h after transfection, and immunoblotting was subsequently performed with the indicated antibodies. Note that the lowest blots were probed with CHIP and GFP antibodies simultaneously. (B) Quantifications of total LRRK2 protein are normalized to expression of β-actin. Statistical significance is indicated relative to WT LRRK2 (n = 3 independent experiments).