Fig. 5.

NAbs prevent viral transfer from carrier cells to target cells by targeting H protein prior to cell-to-cell interaction. Jurkat cells were infected with MV-Edm-GFP at an MOI of 0.5 for 24 h. A These carrier cells were then harvested and incubated with or without NAbs prior to mixing them with A549 cells at a ratio of 1:2 (upper panel), or the Jurkat carrier cells were first mixed with A549 cells at a ratio of 1:2 and NAbs were added after 0.5 or 2 h of co-culture (lower panel). Syncytium formation was monitored by fluorescence microscopy 24 h after mixing. Scale bars represent 80 μm. Representative images from two independent experiments are shown. B The syncytium size was quantified using Adobe Photoshop Software and the mean size in each group is shown. Results are presented as means ± SDs. Similar results were obtained in two independent experiments.