Fig. 4.
Tat1–72 downregulates CREB activity and CREB-mediated gene expression. (A) Primary cultured neurons were treated with 2 μmol/L Tat1–72 for 24 h. Then, the cells were lysed, and the lysate was subjected to western blotting. All data are from three independent experiments. Values are presented as mean ± SEM. **P < 0.01 versus 0 h group. (B) Real-time PCR analysis of CREB expression (relative to GAPDH expression) in cultured neurons treated with Tat1–72 (2 μmol/L) for 6 h. H2O (2.5 μL) used as a control. All data are from three independent experiments. Values are presented as mean ± SEM. **P < 0.01 versus the control group. (C) Real-time PCR analysis of BDNF, c-fos, and Egr-1 expression (relative to GAPDH expression) in cultured neurons treated with Tat1–72 (2 μmol/L) for 24 h. H2O (2.5 μL) used as a control. All data are from three independent experiments. Values are presented as mean ± SEM. **P < 0.01 versus the control group.