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. 2018 Sep 20;11(4):988–997. doi: 10.1016/j.stemcr.2018.08.015

Figure 3.

Figure 3

Flow Cytometry Analysis of the EGFP-Expressing, miPSC-Derived Cells in the Vascular Endothelium and Pericytes of Each Organ

Ratio of donor cell contribution in lung, kidney and aorta of Flk-11173F/1173F or Flk-1+/1173F chimeric mice were analyzed using antibodies against CD45, PECAM1, and PDGFRβ.

(A) Vascular endothelial cells (CD45−/PECAM1+) were gated and analyzed for EGFP expression.

(B) Non-vascular endothelial cells (CD45−/PECAM1−) were gated and analyzed for EGFP expression.

(C) Pericytes (CD45−/PECAM1−/PDGFRβ+) were gated and analyzed for EGFP expression.

Red line, Flk-11173F/1173F chimeric mouse; blue line, Flk-1+/1173F chimeric mouse; gray dashed-line, C57BL/6 mouse shown as a control. Three biological replicates were performed.