Figure 4.

Flow Cytometry Analysis of Chimerism in miPSC-Derived Hematopoietic Cells

(A) Representative flow cytometric plots of EGFP and hematopoietic lineage marker expression in peripheral blood and splenocytes from Flk-1 mutant chimeras. Three biological replicates were performed.

(B) Representative flow cytometric plots and gating for the c-Kit⁺Sca1⁺Lineage⁻ (KSL) fraction within the BM of Flk-1 mutant chimeras. Three biological replicates were performed.

(C) Representative percentage of peripheral blood chimerism of 12 weeks after Flk-1 mutant chimera's BM transplantation into lethally irradiated four recipient mice.

(D) Time course of engraftment of complemented hematopoietic cells are shown. EGFP expression was analyzed in the peripheral blood 4, 8, and 12 weeks after transplantation.

Results are means ± SD of n = 4 (Flk-1^1173F/1173F; 4 weeks), n = 3 (Flk-1^1173F/1173F; 8 weeks), n = 2 (Flk-1^1173F/1173F; 12 weeks), n = 3 (Flk-1^+/1173F; 4 weeks), n = 3 (Flk-1^+/1173F; 8 weeks), and n = 3 (Flk-1^+/1173F; 12 weeks) independent experiments.