FIG 3.

Caspase-1 and NLRP3 in THP-1 cells are involved in T. gondii-mediated IDO1 inhibition. (A and B) WT, CASP1-KO, NLRP1-KO, or NLRP3-KO THP-1 cells were left uninfected or infected with Pru T. gondii. (A) Levels of IL-1β released into the culture supernatant were measured by ELISA. (B) The infected THP-1 cells were cocultured with Huh7 cells and then left untreated or treated with IFN-γ. The parasite survival rates were measured by luciferase assay. Indicated values represent means ± SD (three biological replicates per group from three independent experiments) (panels A and B). ***, P < 0.001; **, P < 0.01; N.S., not significant (Student's t test).