(A) Experimental schematic. Intravascular leukocytes were labeled by injecting BV510-conjugated anti-mouse CD45 antibody intravenously (i.v.) via the retro-orbital venous sinus of naïve C57BL/6 mice 3 min before lung lymphocyte isolation, according to (44).
(B) Representative flow plots (left) and quantification (right) of intravascular-labeled NK cells and ILC1s in blood and lung during homeostasis. NK cells are gated as CD45.2+TCRβ-CD3-NK1.1+CD49b+CD200r1-, and ILC1s as CD45.2+TCRβ-CD3-NK1.1+CD49b-CD200r1+. Data are presented as the mean ± SEM.