Figure 7.
Thermodynamic stability of the mAb and its Fab and Fc fragments in the absence and presence of polysorbates. (a) Chemical denaturant melts with guanidinium chloride (GdmCl) as the denaturant. (b) DSC thermograms. In both panels a and b, black, red, and green curves correspond to that of mAb, Fab, and Fc, respectively. (c-e) Effect of polysorbates on the DSC thermograms of mAb, Fab, and Fc, respectively. In these three panels, black, red, and green curves represent the data in the absence of polysorbates, with PS20, and with PS80, respectively.