Figure 2.
RNF6 regulates gastric cancer cell growth.
Notes: (A, B) shRNF6#1-, shRNF6#2- or shNC-derived lentivirus was infected into NCI-N87 and SNU-1 cells, and cell viability was measured at the indicated times by CCK-8 staining. The expression levels of RNF6 and cyclin D1 were also detected by immunoblotting analysis. GAPDH was used as an internal control. (C, D) NCI-N87 and SNU-1 cells were infected with PLVX-NC- or PLVX-RNF6-derived lentivirus, and cell viability was measured at the indicated times by CCK-8 staining. The expression of RNF6 was also detected by immunoblotting analysis. *P<0.05, **P<0.01.
Abbreviation: CCK-8, Cell Counting Kit-8.