Figure 4.

Knockdown of RNF6 inhibits STAT3 signaling in gastric cancer.

Notes: (A) HGC-27, MGC80-3, NCI-N87 and SNU-1 cells were prepared for immunoblotting against RNF6, p-STAT3 and STAT3. GAPDH was used as an internal control. (B) NCI-N87 and SNU-1 cells transfected with STAT3-Luc were infected with shRNF6#1, shRNF6#2 or shNC for 3 days, and then cells were lysed for luciferase assay. (C) NCI-N87 and SNU-1 cells were infected with shRNF6#1, shRNF6#2 or shNC for 3 days, followed by immunoblotting against RNF6, p-STAT3 and STAT3. GAPDH was used as an internal control. (D, E) shRNF6#1, shRNF6#2 or shNC were infected into NCI-N87 cells. Three days later, cells were prepared for qRT-PCR against MCL1 (D) and XIAP (E). *P<0.01, ^#P<0.01.

Abbreviation: qRT-PCR, quantitative real-time PCR.