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. 2018 Oct 5;11:6589–6597. doi: 10.2147/OTT.S177514

Figure 1.

Figure 1

Expression of Nodal in bladder cancer tissues and cell lines.

Notes: (A) mRNA expression of Nodal in 63 paired bladder cancer tissue samples was analyzed by qRT-PCR. (B) IHC was employed to evaluate the expression of Nodal in bladder cancer tissues and adjacent normal tissues. Magnification: 200× and 400×. (C) Expression of Nodal in the human urothelial cell line (SV-HUC-1) and human bladder cancer cell lines (J82, SW780, 5637, T24, KMBC2, and RT4) was determined by qRT-PCR. (D) Protein expression of Nodal in the human urothelial cell line (SV-HUC-1) and human bladder cancer cell lines (J82, SW780, 5637, T24, KMBC2, and RT4) was tested by Western blot analysis. Data are presented as mean ± SD. *P<0.05 vs SV-HUC-1 cell line. ***P<0.001 vs normal tissue or SV-HUC-1 cell line. Scale bar: 100 µm for 200×, 50 µm for 400×.

Abbreviations: IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction.