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. 2018 Oct 5;11:6589–6597. doi: 10.2147/OTT.S177514

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© 2018 Li et al. This work is published and licensed by Dove Medical Press Limited

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Downregulation of Nodal inhibited cell proliferation, migration, and invasion in bladder cancer cells.

Notes: (A and B) KMBC2 and RT4 cells were transfected with siNodal or siNC plasmids, and transfection efficiencies were determined by qRT-PCR and Western blot. (C) Cell proliferation was evaluated by MTT assay after transfection for 12, 24, 48, and 72 hours. (D) Cell cloning ability of KMBC2 and RT4 cells transfected with siNodal or siNC was determined by colony formation assay. (E and F) Cell migration and invasion were evaluated by transwell assay. Magnification: 200×. (G) Protein expression of CDC6, E-cadherin, MMP-2, and MMP-9 was examined by Western blot analysis. Data are presented as mean ± SD. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs siNC group.

Abbreviation: qRT-PCR, quantitative real-time polymerase chain reaction.