Figure 6.

GSKJ4 restrains the recruitment of JMJD3 to the promoter region of miR-146a. Raw264.7 cells were pre-treated with or without GSKJ4 (4 μmol/L) for 1 h, followed by stimulation with LPS (100 ng/mL) for 24 h. (A,B) ChIP assay was performed using anti-JMJD3, anti-trimethyl H3K27 (H3K27me3), and IgG. The occupancy of each protein was determined with quantitative PCR at the gene promoter region encompassing the JMJD3 binding site. ChIP assay using normal IgG was performed as a negative control. Data are shown as mean ± SEM (n = 3). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.