Figure 8.

miR-146a is transcriptionally co-regulated by JMJD3 and NF-κB p65. Raw264.7 cells were pre-treated with GSKJ4 (4 μmol/L) or BAY11-7082 (5 μmol/L) for 1 h, followed by stimulation with LPS (100 ng/mL) for 24 h. (A–C) ChIP assay was performed using anti-JMJD3, anti-trimethyl H3K27 (H3K27me3), anti-p65, and IgG. The occupancy of each protein was determined with quantitative PCR at the gene promoter region encompassing the JMJD3 or NF-κB binding site. ChIP assay using normal IgG was performed as a negative control. Data are shown as mean ± SEM (n = 3). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.