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. 2018 Feb 9;6(2):e1431038. doi: 10.1080/21688370.2018.1431038

Figure 1.

Figure 1.

Characterization of PMN-derived EVs. (A-B) PMNs were stimulated with fMLF (1μM) to produce EVs. EVs were isolated by serial centrifugation and analyzed by transmission electron microscopy. (A) A representative EV with the size of exosomes (< 100 nm). (B) A representative microparticle/ectosome with the size of ∼600 nm. (C) PMNs (immunolabeled for CD11b, red and myeloperoxidase, green) release myeloperoxidase-containing EVs (shown by arrows) following adhesion to and migration across IECs (surface stain, blue).