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Measurement of cAMP levels with a homogeneous, fluorescent assay. The HEK-293 TSHR cells were seeded to 3 × 104 cells per well and grown overnight. On the x-axis the M22 MAb (0.1 to 100 ng/mL) concentrations were shown in a logarithmic scale and on the y-axis was the relative fluorescence (RF) presented.
