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. 2018 Oct 10;12(10):e0006760. doi: 10.1371/journal.pntd.0006760

Fig 4. Expression pattern of the vas and piwi orthologs in Rhodnius.

Fig 4

A) In situ hybridization assays in early stages of oogenesis (tropharium and vitellarium) for Rp-vas (panel A), Rp-piwi1 (panel B), Rp-piwi2 (panels C and C'), Rp-piwi3 (panel D) and Rp-ago3 (panel E) genes. A sense Rp-ago3 probe was used as control for these assays (panel F). G-H) RT-PCR in previtellogenic stages of oogenesis (panel G) and in mature eggs (panel H) with oligonucleotides specific to Rp-vas, Rp-piwi1, Rp-piwi2, Rp-piwi3 and Rp-ago3 genes.