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. 2018 Oct 10;13(10):e0203936. doi: 10.1371/journal.pone.0203936

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© 2018 Caseiro et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 11 — Multilineage differentiation: A) Osteogenic differentiation: Alizarin Red S concentration (μM) after 21 days; B) Adipogenic differentiation: Oil Red O (OD_570nm) after 14 days; and C) Chondrogenic differentiation: Sulfated GAGs production (μg/mL) after 14 days, assessed by Blyscan™ Glycosaminoglycan Assay (Biocolor, UK). Control: Undifferentiated control; Results Presented as Mean ± SEM. a: significantly different from undifferentiated group within the same media condition and cellular population; b: undifferentiated control group significantly different from undifferentiated FBS 10% group, within the cellular population; c: differentiated group significantly different from differentiated FBS 10%, within the cellular population.