Fig 6. Evidence that Dynasore protects the cell surface glycocalyx against damage due to oxidative stress.

HCLE stratified cultures plated in triplicate were either left unstressed or stressed with 3 mM tBHP. At the same time, a set of unstressed and stressed cultures were treated with Dynasore (40 uM). (An equal volume of the Dynasore diluent, DMSO, was added to untreated cultures.) After 2 hours, (A) the cells were stained with a lectin, fluorescein-conjugated jacalin and imaged under epifluorescent illumination. Representative images are shown from each triplicate set. (B) Proteins in the media were resolved by SDS-PAGE, western blotted, and probed with an antibody specific to LGALS3 (top). DNA in the medium was also subjected to genomic PCR for ACTB gene detection (bottom).