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. 2018 Oct 10;13(10):e0205344. doi: 10.1371/journal.pone.0205344

Fig 8. Activation of S1PR1 blocked p65 from translocation into the nucleus.

Fig 8

HPMECs were transfected with the plasmid expressing HA-tagged S1PR1 and treated with CYM5442 prior to the IAV H9N2 infection. At 24 hrs post infection, the cells were stained with an anti-NF-κB p65 antibody and subsequently a secondary antibody. Nuclear translocation of NF-κB p65 was examined by confocal microscopy. (A) non-infected HPMECs; (B) H9N2-infected HPMECs; (C) HPMECs, transfected with S1PR1 plasmid and treated with CYM5442, and subsequently infected with the IAV H9N2. Scale bar, 50μm.