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. 2018 Oct 10;13(10):e0205520. doi: 10.1371/journal.pone.0205520

Fig 1. FRAP measurements of DiOC18 in the plasma membrane of HeLa cells treated with DMSO and ethylene glycol or enriched with cholesterol.

Fig 1

HeLa cells have been labeled with DiOC18. They have been kept in phosphate buffered medium (control), treated with a combination of 15% DMSO and 15% ethylene glycol (DE-medium) or enriched with cholesterol by treating them with 10 mM cholesterol-loaded methyl-β-cyclodextrin for 60 min (Cholesterol). A section of the plasma membrane (app. 2 μm) has been bleached and the fluorescence recovery has been recorded for 100 s. From this data, the immobile fraction (a) and the diffusion speed of the mobile fraction (b) have been calculated. Data is shown as single cell measurements (gray dots) and mean +/- s.d. (black bars); n = 10 (control); n = 22 (DE-medium); n = 19 (Cholesterol); ***: p<0.001; **:p<0.01 using parametric one-way ANOVA and Tukey post-hoc test.