Table 1.

Analysis of lifespan

Sex	Temperature	Direction	χ2	P	n
Female	18°C	FI < FS	35.86	<0.0001†	2609 (2639)
		FI < MS	137.31	<0.0001†	2591 (2644)
		FS < MS	28.52	<0.0001†	2520 (2571)
	25°C	FI < FS	98.75	<0.0001†	2500 (2549)
		FI < MS	71.53	<0.0001†	2478 (2539)
		FS = MS	0.65	0.42	2508 (2566)
Male	18°C	FI < FS	85.72	<0.0001†	2437 (2455)
		FI < MS	247.67	<0.0001†	2514 (2547)
		FS < MS	37.26	<0.0001†	2453 (2484)
	25°C	FI < FS	100.39	<0.0001†	2565 (2600)
		FI < MS	109.59	<0.0001†	2537 (2580)
		FS = MS	0.32	0.57	2512 (2558)

The columns show the directionality of lifespan effects for each pairwise comparison between the three karyotypes (FI = Florida inverted, FS = Florida standard, MS = Maine standard), grouped by sex and temperature. χ² test statistics and P-values are from generalized Wilcoxon tests. Significant effects are in bold; significance after Bonferroni correction is indicated by † (α′= 0.05/3 = 0.016). n represents the number of dead individuals; the total cohort size is shown in parenthesis. See Results and Figs. 1 and S1 for further details.