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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Evol Biol. 2018 Jun 28;31(9):1354–1364. doi: 10.1111/jeb.13310

Table 3.

Analysis of survival upon cold shock.

Sex Temperature Direction χ2 P n
Female 18°C FI = FS 0.64 0.43 197 (887)
FI = MS 0.88 0.35 199 (888)
FS = MS 0.02 0.89 210 (893)

25°C FI < FS 47.39 <0.0001 694 (897)
FI < MS 77.48 <0.0001 664 (896)
FS < MS 4.18 0.04 580 (899)

Male 18°C - - - 0 (896)
- - - 0 (897)
- - - 0 (895)

25°C FI = FS 2.01 0.16 737 (887)
FI = MS 2.35 0.14 734 (885)
FS = MS 0.01 0.90 719 (886)

The columns show the directionality of survival upon cold shock for each pairwise comparison between the three karyotypes (FI = Florida inverted, FS = Florida standard, MS = Maine standard), grouped by sex and temperature. χ2 test statistics and P-values are from generalized Wilcoxon tests. Significant effects are in bold; significance after Bonferroni correction is indicated by † (α′ = 0.05/3 = 0.016). n represents the number of dead individuals; the total cohort size is shown in parenthesis. Note that at 18°C all males survived 24 hours of cold shock and were thus all censored. See Results and Fig. 3 for further details.