Table 1.
Primer Information
| Annealing | Efficiency | |||||||
|---|---|---|---|---|---|---|---|---|
| Gene name (encodes for) | Accession number | Forward primer location (nt) | Reverse primer location (nt) | Product length (bp) | Temperature (°) | Slope | r 2 | % |
| Pomc (POMC)a | NM_008895 | 145-164 | 327-344 | 200 | 60.5 | |||
| Npy (NPY)a | NM_023456 | 106-125 | 268-287 | 182 | 60 | |||
| Agrp (AgRP)a | NM_001271806 | 397-418 | 532-542 | 146 | 59 | |||
| Oprm1 (muOR)a | NM_001302793 | 518-537 | 602-619 | 102 | 57 | |||
| Slc17a6 (vGluT2)a | NM_080853 | 1038-1056 | 1213-1231 | 194 | 57 | |||
| Slc17a6 (vGluT2)b | NM_080853 | 872-889 | 967-984 | 113 | 60 | -3.293 | 0.92 | 100 |
| Slc32a1 (vGAT)a | NM_009508 | 813-834 | 928-949 | 137 | 60 | |||
| Kiss1 (Kiss1)a | NM_178260 | 64-80 | 167-183 | 120 | 57 | |||
| Slc18a2 (VMAT2)b | NM_172523 | 1021-1041 | 1123-1143 | 123 | 60 | -3.328 | 0.864 | 100 |
| Actb (β-actin)b | NM_007393 | 446-465 | 535-555 | 110 | 60 | -3.465 | 0.996 | 95 |
a
primers used for scRT-PCR.
b
primers used for qPCR.