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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Immunol. 2018 Aug 31;201(8):2492–2501. doi: 10.4049/jimmunol.1800003

Figure 1. Neutrophil conditioned media induces AREG expression by IECs.

Figure 1.

Intestinal enteroids were cultured with or without neutrophil conditioned media for 6 h followed by microarray analysis. (A) Hierarchical clustering of genes that were differentially (|Fold change| > 2) expressed between the two groups (n = 2). Heat map of some differentially expressed probes between the two groups (n = 2). (B) Scatterplot displaying the log2 fold change in expression between the two groups (n = 2). (C) mRNA expression levels of AREG in the enteroids were measured by qRT-PCR and normalized to GAPDH. (D) Primary IECs from either small intestines (SB) or large intestines (LB) and treated with or without neutrophil conditioned media for 6 h, and mRNA expression level of AREG measured by qRT-PCR and normalized to GAPDH. (E) mRNA expression level of AREG in the MSIE cells treated with neutrophil conditioned media (CM) for 6 h. Data are presented as mean ± SEM of three independent experiments. CM, neutrophil conditioned media; None, media alone. *P < 0.05, **P < 0.01 Student’s t test.

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