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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Immunol. 2018 Aug 31;201(8):2492–2501. doi: 10.4049/jimmunol.1800003

Figure 4. TGFβ induces AREG production in IEC.

Figure 4.

(A) MSIE cells were treated with different cytokines/factors for 6 h, and mRNA expression level of AREG was measured by qRT-PCR and normalized against GAPDH. (B) Level of TGFβ was determined in neutrophil conditioned media versus media control. (C) mRNA expression level of AREG in the MSIE cells treated with 20 μg/ml TGFβ over time, determined by qRT-PCR and normalized against GAPDH. (D and E) Neutrophil conditioned media was pre-treated with 10 μg/ml anti-TGFβ antibody and then added into MSIE cell cultures. (D) mRNA expression level (24 h) and (E) protein level of AREG (24 h) were measured by qRT-PCR and Western blot, respectively. (F) mRNA expression level of AREG in primary IECs isolated from either SB or LB. Data are presented as mean ± SEM of three independent experiments. CM, neutrophil conditioned media; None, media alone. *P < 0.05, **P < 0.01, ***P < 0.001 Student’s t test, one-way ANOVA.

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