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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Immunol. 2018 Aug 31;201(8):2492–2501. doi: 10.4049/jimmunol.1800003

Figure 5. Neutrophils produce TGFβ which regulates IEC production of AREG in the setting of inflammation.

Figure 5.

(A) mRNA expression level of TGFβ in bone marrow, peritoneal cavity, spleen neutrophils, with Treg as positive control. (B) Protein levels of TGFβ in the colonic tissue culture were analyzed by ELISA. (C) Soluble AREG was measured in colonic culture supernatant via ELISA. (D and E) IECs were isolated from the colon of mice, mRNA expression level (G) and protein level (E) of AREG were measured in IECs. (F) mRNA expression level of ADAM17 in the colonic IECs of mice. (G) Intestinal enteroids were cultured with or without neutrophil conditioned media for 6 h, mRNA expression levels of ADAM17 in the enteroids were measured by qRT-PCR and normalized to GAPDH. Data are representative of 3 independent experiments; PMN, neutrophil; *P < 0.05, **P < 0.01 unpaired Student’s t test, one-way ANOVA, nonparametric Mann–Whitney U test.

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